Development of Effective Protocol for Four Varieties of Large Cardamom

Large cardamom is one of the most important spices that can significantly contribute to economical farming in country Nepal. It grown Nepal and north-eastern states India which provide suitable agroclimatic growing conditions high humidity, ambient temperature rainfall. Meeting demand for quality plants yield challenging with traditional methods propagation. The present study has used plant tissue culture technique produce plants. In this regard, MS media three different hormonal combinations were development protocol 8 weeks. Shoot length, root shoot number assessed at intervals. best growth was 1 mg/L BAP + 0.5 IBA Ramsey variety, no significant difference Golsai, Dambarsai, or Sikkimae varieties. Similarly, acclimatization field transfer done. use any substrate composition ratio coco peat: soil 1:2; moss: peat 1:2 sawdust: enables healthy field. results indicate varieties respond differently micropropagation process hormone concentrations indicated by differing production. 1mg/L 0.5mg/L could be Ramsai while optimal production Golsai should 5mg/L All showed 0mg/L IBA. This sheds light on responsiveness both micropropagation.